Analyses of human papillomavirus genotypes and viral loads in anogenital warts
Identifieur interne : 000042 ( France/Analysis ); précédent : 000041; suivant : 000043Analyses of human papillomavirus genotypes and viral loads in anogenital warts
Auteurs : Siolian L. R. Ball [Royaume-Uni] ; David M. Winder [Royaume-Uni] ; Katie Vaughan [Royaume-Uni] ; Nashat Hanna [Royaume-Uni] ; Jonathan Levy [France] ; Jane C. Sterling [Royaume-Uni] ; Margaret A. Stanley [Royaume-Uni] ; Peter K. C. Goon [Royaume-Uni]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2011-08.
English descriptors
- Teeft :
- Anogenital, Anogenital warts, Assay, Bakkers jmje, Cervical, Clin, Condyloma, Condylomata acuminata, Copy number, Genital, Genital wart samples, Genital warts, Genotyping test, High risk, High risk hpvs, Hpvs, Human papillomavirus, Human papillomavirus infection, Human papillomavirus types, Lesion, Lesion causality, Linear array, Linear array assay, Linear array results, Massuger lfag, Methods section, Mrna, Mrna analysis, Mrna levels, Multiple infections, Oncogenic hpvs, Other hpvs, Papillomavirus, Primer, Qpcr, Relative mrna levels, Risk hpvs, Standard curves, Strain prevalence, Supplementary table, Taqmantm qpcr, Threshold value, Vaccine, Vaccine failure, Viral, Viral load, Viral loads, Virol, Wart tissue, Young women.
Abstract
Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this, the strain prevalence and the frequency of multiple HPV infections were determined in wart tissue obtained from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV‐type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV 6, 11, 16, and 18 viral loads and RT‐qPCR measured HPV 6 and 11 E6 genomic expression levels. Seventy‐one percent of these samples were infected with multiple HPVs. Only one sample was negative for HPV 6 or 11 DNA. Forty‐eight percent of samples were positive for a high risk (oncogenic) HPV. The results show that multiple infections in tissue are frequent and the subsequent analysis of HPV 6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV 6/11 E6 mRNA levels showed that there was no discernable relationship between HPV 6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality. J. Med. Virol. 83:1345–1350, 2011. © 2011 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.22111
Affiliations:
- France, Royaume-Uni
- Angleterre, Angleterre de l'Est, Grand Londres, Île-de-France
- Cambridge, Londres, Paris
- Université de Cambridge
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000617
- to stream Istex, to step Curation: 000597
- to stream Istex, to step Checkpoint: 000098
- to stream Main, to step Merge: 000C93
- to stream Main, to step Curation: 000C91
- to stream Main, to step Exploration: 000C91
- to stream France, to step Extraction: 000042
Links to Exploration step
ISTEX:4D122193585FC84E1D55726EC916A2AC11A358EFLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analyses of human papillomavirus genotypes and viral loads in anogenital warts</title><author><name sortKey="Ball, Siolian L R" sort="Ball, Siolian L R" uniqKey="Ball S" first="Siolian L. R." last="Ball">Siolian L. R. Ball</name></author><author><name sortKey="Winder, David M" sort="Winder, David M" uniqKey="Winder D" first="David M." last="Winder">David M. Winder</name></author><author><name sortKey="Vaughan, Katie" sort="Vaughan, Katie" uniqKey="Vaughan K" first="Katie" last="Vaughan">Katie Vaughan</name></author><author><name sortKey="Hanna, Nashat" sort="Hanna, Nashat" uniqKey="Hanna N" first="Nashat" last="Hanna">Nashat Hanna</name></author><author><name sortKey="Levy, Jonathan" sort="Levy, Jonathan" uniqKey="Levy J" first="Jonathan" last="Levy">Jonathan Levy</name></author><author><name sortKey="Sterling, Jane C" sort="Sterling, Jane C" uniqKey="Sterling J" first="Jane C." last="Sterling">Jane C. Sterling</name></author><author><name sortKey="Stanley, Margaret A" sort="Stanley, Margaret A" uniqKey="Stanley M" first="Margaret A." last="Stanley">Margaret A. Stanley</name></author><author><name sortKey="Goon, Peter K C" sort="Goon, Peter K C" uniqKey="Goon P" first="Peter K. C." last="Goon">Peter K. C. Goon</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4D122193585FC84E1D55726EC916A2AC11A358EF</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1002/jmv.22111</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-PJRJLHR6-H/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000617</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000617</idno><idno type="wicri:Area/Istex/Curation">000597</idno><idno type="wicri:Area/Istex/Checkpoint">000098</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000098</idno><idno type="wicri:doubleKey">0146-6615:2011:Ball S:analyses:of:human</idno><idno type="wicri:Area/Main/Merge">000C93</idno><idno type="wicri:Area/Main/Curation">000C91</idno><idno type="wicri:Area/Main/Exploration">000C91</idno><idno type="wicri:Area/France/Extraction">000042</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Analyses of human papillomavirus genotypes and viral loads in anogenital warts</title><author><name sortKey="Ball, Siolian L R" sort="Ball, Siolian L R" uniqKey="Ball S" first="Siolian L. R." last="Ball">Siolian L. R. Ball</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Royaume-Uni</country></affiliation><affiliation wicri:level="4"><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>Correspondence address: Division of Virology, Department of Pathology, University of Cambridge, Level 5 Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Winder, David M" sort="Winder, David M" uniqKey="Winder D" first="David M." last="Winder">David M. Winder</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Vaughan, Katie" sort="Vaughan, Katie" uniqKey="Vaughan K" first="Katie" last="Vaughan">Katie Vaughan</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Hanna, Nashat" sort="Hanna, Nashat" uniqKey="Hanna N" first="Nashat" last="Hanna">Nashat Hanna</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of GU/HIV Medicine, St. Mary's Hospital, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Levy, Jonathan" sort="Levy, Jonathan" uniqKey="Levy J" first="Jonathan" last="Levy">Jonathan Levy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Paris Diderot University, ERAMUS Scholar 2007, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Sterling, Jane C" sort="Sterling, Jane C" uniqKey="Sterling J" first="Jane C." last="Sterling">Jane C. Sterling</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Stanley, Margaret A" sort="Stanley, Margaret A" uniqKey="Stanley M" first="Margaret A." last="Stanley">Margaret A. Stanley</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Goon, Peter K C" sort="Goon, Peter K C" uniqKey="Goon P" first="Peter K. C." last="Goon">Peter K. C. Goon</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pathology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">83</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1345">1345</biblScope><biblScope unit="page" to="1350">1350</biblScope><biblScope unit="page-count">6</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-08">2011-08</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Anogenital</term><term>Anogenital warts</term><term>Assay</term><term>Bakkers jmje</term><term>Cervical</term><term>Clin</term><term>Condyloma</term><term>Condylomata acuminata</term><term>Copy number</term><term>Genital</term><term>Genital wart samples</term><term>Genital warts</term><term>Genotyping test</term><term>High risk</term><term>High risk hpvs</term><term>Hpvs</term><term>Human papillomavirus</term><term>Human papillomavirus infection</term><term>Human papillomavirus types</term><term>Lesion</term><term>Lesion causality</term><term>Linear array</term><term>Linear array assay</term><term>Linear array results</term><term>Massuger lfag</term><term>Methods section</term><term>Mrna</term><term>Mrna analysis</term><term>Mrna levels</term><term>Multiple infections</term><term>Oncogenic hpvs</term><term>Other hpvs</term><term>Papillomavirus</term><term>Primer</term><term>Qpcr</term><term>Relative mrna levels</term><term>Risk hpvs</term><term>Standard curves</term><term>Strain prevalence</term><term>Supplementary table</term><term>Taqmantm qpcr</term><term>Threshold value</term><term>Vaccine</term><term>Vaccine failure</term><term>Viral</term><term>Viral load</term><term>Viral loads</term><term>Virol</term><term>Wart tissue</term><term>Young women</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this, the strain prevalence and the frequency of multiple HPV infections were determined in wart tissue obtained from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV‐type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV 6, 11, 16, and 18 viral loads and RT‐qPCR measured HPV 6 and 11 E6 genomic expression levels. Seventy‐one percent of these samples were infected with multiple HPVs. Only one sample was negative for HPV 6 or 11 DNA. Forty‐eight percent of samples were positive for a high risk (oncogenic) HPV. The results show that multiple infections in tissue are frequent and the subsequent analysis of HPV 6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV 6/11 E6 mRNA levels showed that there was no discernable relationship between HPV 6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality. J. Med. Virol. 83:1345–1350, 2011. © 2011 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Grand Londres</li><li>Île-de-France</li></region><settlement><li>Cambridge</li><li>Londres</li><li>Paris</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Ball, Siolian L R" sort="Ball, Siolian L R" uniqKey="Ball S" first="Siolian L. R." last="Ball">Siolian L. R. Ball</name></region><name sortKey="Ball, Siolian L R" sort="Ball, Siolian L R" uniqKey="Ball S" first="Siolian L. R." last="Ball">Siolian L. R. Ball</name><name sortKey="Ball, Siolian L R" sort="Ball, Siolian L R" uniqKey="Ball S" first="Siolian L. R." last="Ball">Siolian L. R. Ball</name><name sortKey="Goon, Peter K C" sort="Goon, Peter K C" uniqKey="Goon P" first="Peter K. C." last="Goon">Peter K. C. Goon</name><name sortKey="Hanna, Nashat" sort="Hanna, Nashat" uniqKey="Hanna N" first="Nashat" last="Hanna">Nashat Hanna</name><name sortKey="Stanley, Margaret A" sort="Stanley, Margaret A" uniqKey="Stanley M" first="Margaret A." last="Stanley">Margaret A. Stanley</name><name sortKey="Sterling, Jane C" sort="Sterling, Jane C" uniqKey="Sterling J" first="Jane C." last="Sterling">Jane C. Sterling</name><name sortKey="Vaughan, Katie" sort="Vaughan, Katie" uniqKey="Vaughan K" first="Katie" last="Vaughan">Katie Vaughan</name><name sortKey="Winder, David M" sort="Winder, David M" uniqKey="Winder D" first="David M." last="Winder">David M. Winder</name></country><country name="France"><region name="Île-de-France"><name sortKey="Levy, Jonathan" sort="Levy, Jonathan" uniqKey="Levy J" first="Jonathan" last="Levy">Jonathan Levy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000042 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000042 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:4D122193585FC84E1D55726EC916A2AC11A358EF
|texte= Analyses of human papillomavirus genotypes and viral loads in anogenital warts
}}
| This area was generated with Dilib version V0.6.33. |  |